10 fewer sets in 4DN-only Data.
Included Properties
Data Type
Dataset
Sample Type
Sample Category
Sample
Organ/System
Tissue Source
Treatments
Assay Details
Set Type
Warnings
11 Common Properties
Project
External
Lab
Alistair Boettiger, STANFORD
Experiment Category
Microscopy
Experiment Type
FISH
Organism
Human
Sample Tier
Unclassified
Publication
Bintu B et al. (2018) PMID:30361340
Modifications
No value
Center
External
Status
Released
Commendations
No value
Title
Experiment Type
Biosample
Dataset
Condition
Assay Details
Lab
multiplexed FISH
HCT116 with Crispr generated mClover and AID-tagged RAD21
Chromatin tracing of 2-Mb on chr21
Chr21:34-37 Mb in HCT116 cells cohesin-depleted after cell division
Target
83 consecutive 30-kb loci on chr21, Geminin, EdU labeled Chromosomes
multiplexed FISH
HCT116 with Crispr generated mClover and AID-tagged RAD21
Chromatin tracing of 2-Mb on chr21
Chr21:34-37 Mb in HCT116 cells cohesin-depleted
Target
83 consecutive 30-kb loci on chr21
multiplexed FISH
HCT116 with Crispr generated mClover and AID-tagged RAD21
Chromatin tracing of 2-Mb on chr21
Chr21:34-37 Mb in HCT116 cells
Target
83 consecutive 30-kb loci on chr21
multiplexed FISH
HCT116 with Crispr generated mClover and AID-tagged RAD21
Chromatin tracing of 2-Mb on chr21
Chr21:28-30 Mb in HCT116 cells cohesin-depleted
Target
65 consecutive 30-kb loci on chr21
multiplexed FISH
HCT116 with Crispr generated mClover and AID-tagged RAD21
Chromatin tracing of 2-Mb on chr21
Chr21:28-30 Mb in HCT116 cells
Target
65 consecutive 30-kb loci on chr21
multiplexed FISH
A549
Chromatin tracing of 2-Mb on chr21
Chr21:28-30 Mb in A549 cells
Target
65 consecutive 30-kb loci on chr21
multiplexed FISH
K562
Chromatin tracing of 2-Mb on chr21
Chr21:28-30 Mb in K562 cells
Target
65 consecutive 30-kb loci on chr21
multiplexed FISH
IMR-90
Chromatin tracing of 2-Mb on chr21
Chr21:28-30 Mb in IMR90 cells across cell cycle
Target
65 consecutive 30-kb loci on chr21, Geminin, Chromosomes
multiplexed FISH
IMR-90
Chromatin tracing of 2-Mb on chr21
Chr21:28-30 Mb in IMR90 cells
Target
65 consecutive 30-kb loci on chr21
multiplexed FISH
IMR-90
Chromatin tracing of 2-Mb on chr21
Chr21:18-20 Mb (no TADs) in IMR90 cells
Target
65 consecutive 30-kb loci in a region without TADs on chr21
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